Journal: Pediatric Reports

Article Title: Resistin in Urine and Breast Milk: Relation to Type of Feeding and Anthropometry at 1-Month

doi: 10.3390/pediatric14010013

Figure Lengend Snippet: Correlation between resistin concentration in the breast milk and ( a ) body weight; ( b ) age; ( c ) body mass index (BMI); and ( d ) leptin concentration in mothers’ breast milk at 1 month post-partum.

Article Snippet: We analyzed urinary and breast milk resistin levels (Human Resistin ELISA kit; Arigo biolaboratories, Hsinchu City, Taiwan) and breastmilk leptin (Human Leptin Quantikine ELISA Kit; R&D Systems, Minneapolis, MI, USA) in infants using an enzyme-linked immunosorbent assay kit.

Techniques: Concentration Assay

Journal: Biomolecules

Article Title: Adipocytokines in Untreated Newly Diagnosed Rheumatoid Arthritis: Association with Circulating Chemokines and Markers of Inflammation

doi: 10.3390/biom11020325

Figure Lengend Snippet: Levels of plasma adipocytokines and chemokines.

Article Snippet: Total and HMW adiponectin, leptin, and resistin in plasma were measured using the following enzyme-linked immunosorbent assays (ELISA): Human Total Adiponectin/Acrp30 Quantikine ELISA, Human HMW Adiponectin/Acrp30 Quantikine ELISA, Human Leptin Quantikine ELISA, and Human Resistin Quantikine ELISA (R&D Systems, Bio-Techne, Minneapolis, MN, USA).

Techniques: Clinical Proteomics

Journal: PLOS One

Article Title: Metabolomics and glucose tolerance in pregnancy and postpartum: The PONCH study

doi: 10.1371/journal.pone.0335708

Figure Lengend Snippet: Selected clinical measurements (A) and metabolites (B) are compared between NW (n = 33), OB (n = 32), GDM-NW (n = 15) and GDM-OB (n = 16) women. The boxes represent the 25 th to 75 th percentiles with Tukey whiskers and the line indicating median. *p < 0.05, **p < 0.01, ***p < 0.001 and ****p < 0.0001 for group comparisons using Kruskal-Wallis analysis and Mann-Whitney-U statistics adjusted for multiple comparisons. Observed power for Kruskal-Wallis analysis was ≥ 0.8 for all variables except one (Valine, power = 0.4) C) Overview of significant differences. NW, women of normal weight; OB, women with obesity; GDM-NW, women with GDM and of normal weight; GDM-OB, women with GDM and obesity; LepR, soluble leptin receptor; HOMA-IR, homeostatic model assessment of insulin resistance; BCAA, branched chained amino acids.

Article Snippet: Leptin (Human Leptin Quantikine, R&D Systems, Minneapolis, MN; interassay coefficient of variation, 8.0% at 9 μg/l), the soluble leptin receptor (LepR) (Human Leptin R Quantikine, R&D Systems) and adiponectin (Human Adiponectin ELISA kit, Millipore, Billerica, MA; interassay coefficient of variation, 7.0% at 10.5 mg/l) were analysed simultaneously for all samples using ELISA.

Techniques: MANN-WHITNEY

Journal: Diabetes

Article Title: Adipocyte lipases and defect of lipolysis in human obesity.

doi: 10.2337/diabetes.54.11.3190

Figure Lengend Snippet: FIG. 5. Lipolysis, leptin secretion, and HSL expression in mature adipocytes and differentiated preadipocytes from obese and lean sub- jects. A: Glycerol and leptin release in isolated mature adipocytes from 42 lean and 81 obese subjects. B: Glycerol and leptin release in preadipocytes differentiated in primary culture from 42 lean and 81 obese subjects. C: Protein expression of HSL and 2-adrenoceptor (2-AR) in differentiated human preadipocytes from 14 obese and 7 lean subjects determined by Western blot analysis.

Article Snippet: Leptin in the medium was measured using Quantikine Human Leptin Immunoassay (R&D Systems).

Techniques: Expressing, Isolation, Western Blot

Journal: American Journal of Human Biology

Article Title: Validation of an Enzyme‐Linked Immunosorbent Assay for Measuring Leptin, a Key Metabolic Hormone, in Dried Blood Spot Samples

doi: 10.1002/ajhb.70172

Figure Lengend Snippet: Passing–Bablok Regression for leptin measurement in matched DBS and serum samples ( n = 40). Results demonstrate strong agreement between leptin measurements in DBS and serum.

Article Snippet: The Human Leptin Quantikine QuicKit ELISA (R&D Systems, QK398) was validated using matched serum and DBS samples from 40 adults.

Techniques:

Journal: American Journal of Human Biology

Article Title: Validation of an Enzyme‐Linked Immunosorbent Assay for Measuring Leptin, a Key Metabolic Hormone, in Dried Blood Spot Samples

doi: 10.1002/ajhb.70172

Figure Lengend Snippet: Bland Altman plot of the difference in serum leptin and DBS leptin (serum‐equivalent) values vs. the average of these values. Results demonstrate general agreement between both measures, with no apparent bias.

Article Snippet: The Human Leptin Quantikine QuicKit ELISA (R&D Systems, QK398) was validated using matched serum and DBS samples from 40 adults.

Techniques: